Tomas Malinauskas
Contact information
tomas@strubi.ox.ac.uk
tomas.malinauskas@gmail.com
https://orcid.org/0000-0002-4847-5529
Wellcome Centre for Human Genetics, Roosevelt Drive, Oxford, OX3 7BN, United Kingdom
Research groups
Collaborators
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E. Yvonne Jones
The Sir Andrew McMichael Professor of Structural Immunology
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David Stuart
Professor of Structural Biology
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Loic Carrique
Senior CryoEM Staff Scientist
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Karl Harlos
Post Doctoral Research Associate
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Tom Walter
Post Doctoral Research Associate
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Margaret Jones
Laboratory Manager
Tomas Malinauskas
Post Doctoral Research Associate
I am a structural biologist in Prof. Christian Siebold's group at the University of Oxford, UK. I use biochemical (mammalian cell-based protein expression, chromatography, cellular assays), biophysical (X-ray crystallography, cryo-EM, SPR, BLI, MALS) and computational (homology modelling, small molecule docking, evolutionary conservation analysis) tools to elucidate the molecular mechanisms of cell-to-cell communication. I aim to understand the way these mechanisms create and maintain living organisms.
If you need a structural perspective on your project, please email me. I am happy to help with anything from advice on protein constructs and expression to analysis of the target protein structure-function (e.g. the effect of mutations) to structural figures and movies for your papers.
Selected publications:
1. Robinson, R.A.*, Griffiths, S.C.*, van de Haar, L.L.*, Malinauskas, T.*, et al. Simultaneous binding of Guidance Cues NET1 and RGM blocks extracellular NEO1 signaling. Cell, 184, 1–18 (2021).
2. Malinauskas, T.**, et al. Repulsive Guidance Molecules lock Growth Differentiation Factor 5 in an inhibitory complex. Proc. Natl. Acad. Sci. U.S.A., 117 (27), 15620–15631 (2020).
3. Zhao, Y.*, Malinauskas, T.*, et al. Structural insights into the inhibition of Wnt signaling by cancer antigen 5T4/Wnt-Activated Inhibitory Factor 1. Structure, 22 (4), 612–620 (2014).
4. Janssen, B.J.C.*, Malinauskas, T.*, et al. Neuropilins lock secreted semaphorins onto plexins in a ternary signalling complex. Nat. Struct. Mol. Biol., 19 (12), 1293–1299 (2012).
5. Malinauskas, T., et al. Modular mechanism of Wnt signaling inhibition by Wnt inhibitory factor 1. Nat. Struct. Mol. Biol., 18 (8), 886–893 (2011).
*Equal contribution.
**Co-corresponding author.
Websites
Recent publications
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GWAS and meta-analysis identifies 49 genetic variants underlying critical COVID-19.
Journal article
Pairo-Castineira E. et al, (2023), Nature
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Whole-genome sequencing reveals host factors underlying critical COVID-19.
Journal article
Kousathanas A. et al, (2022), Nature, 607, 97 - 103
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Differential assembly diversifies GABAA receptor structures and signalling.
Journal article
Sente A. et al, (2022), Nature, 604, 190 - 194
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Hedgehog-Interacting Protein is a multimodal antagonist of Hedgehog signalling
Journal article
Griffiths SC. et al, (2021), Nature Communications, 12
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Anti-prothrombin autoantibodies enriched after infection with SARS-CoV-2 and influenced by strength of antibody response against SARS-CoV-2 proteins.
Journal article
Emmenegger M. et al, (2021), PLoS pathogens, 17
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A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses
Journal article
Tan TK. et al, (2021), Nature Communications, 12
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Structure dynamics of HIV-1 Env trimers on native virions engaged with living T cells.
Journal article
Carlon-Andres I. et al, (2021), Communications biology, 4
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Site-Specific Steric Control of SARS-CoV-2 Spike Glycosylation.
Journal article
Allen JD. et al, (2021), Biochemistry, 60, 2153 - 2169
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Site-specific steric control of SARS-CoV-2 spike glycosylation.
Journal article
Allen JD. et al, (2021), bioRxiv
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Author Correction: Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2
Journal article
Huo J. et al, (2021), Nature Structural & Molecular Biology, 28, 326 - 326
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Author Correction: Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2
Journal article
Huo J. et al, (2020), Nature Structural & Molecular Biology, 27, 1094 - 1094
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Single-particle cryo-EM at atomic resolution.
Journal article
Nakane T. et al, (2020), Nature, 587, 152 - 156
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Structural basis for the neutralization of SARS-CoV-2 by an antibody from a convalescent patient
Journal article
Zhou D. et al, (2020), Nature Structural & Molecular Biology, 27, 950 - 958
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Glypicans shield the Wnt lipid moiety to enable signalling at a distance.
Journal article
McGough IJ. et al, (2020), Nature, 585, 85 - 90
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Neutralization of SARS-CoV-2 by Destruction of the Prefusion Spike
Journal article
Huo J. et al, (2020), Cell Host & Microbe, 28, 445 - 454.e6
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Neutralization of SARS-CoV-2 by Destruction of the Prefusion Spike
Journal article
Huo J. et al, (2020), Cell Host & Microbe, 28, 497 - 497
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Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2
Journal article
Huo J. et al, (2020), Nature Structural & Molecular Biology, 27, 846 - 854
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Repulsive guidance molecules lock growth differentiation factor 5 in an inhibitory complex.
Journal article
Malinauskas T. et al, (2020), Proceedings of the National Academy of Sciences of the United States of America, 117, 15620 - 15631
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Single-particle cryo-EM at atomic resolution
Preprint
Nakane T. et al, (2020)
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R-spondins engage heparan sulfate proteoglycans to potentiate WNT signaling.
Journal article
Dubey R. et al, (2020), eLife, 9
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Diversity of oligomerization in Drosophila semaphorins suggests a mechanism of functional fine-tuning
Journal article
Rozbesky D. et al, (2019), Nature Communications, 10
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The morphogen Sonic hedgehog inhibits its receptor Patched by a pincer grasp mechanism.
Journal article
Rudolf AF. et al, (2019), Nature chemical biology, 15, 975 - 982
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Author Correction: GABAA receptor signalling mechanisms revealed by structural pharmacology.
Journal article
Masiulis S. et al, (2019), Nature, 566
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Cryo-EM structure of the human α1β3γ2 GABAA receptor in a lipid bilayer.
Journal article
Laverty D. et al, (2019), Nature, 565, 516 - 520
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GABAA receptor signalling mechanisms revealed by structural pharmacology.
Journal article
Masiulis S. et al, (2019), Nature, 565, 454 - 459
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Calcium-sensing receptor residues with loss- and gain-of-function mutations are located in regions of conformational change and cause signalling bias
Journal article
Gorvin CM. et al, (2018), Human Molecular Genetics, 27, 3720 - 3733
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A calcium-sensing receptor mutation causing hypocalcemia disrupts a transmembrane salt bridge to activate β-arrestin–biased signaling
Journal article
Gorvin CM. et al, (2018), Science Signaling, 11
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Structural Basis for Growth Differentiation Factor 5 (GDF5) Signaling Inhibition by Repulsive Guidance Molecules (RGMs)
Conference paper
Malinauskas T. et al, (2018), BIOPHYSICAL JOURNAL, 114, 464A - 464A
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A Calcium-Sensing Receptor (CASR) Mutation Causes Hypocalcemia by Disrupting a Transmembrane Salt Bridge that Modulates beta-arrestin Signaling.
Conference paper
Gorvin C. et al, (2017), JOURNAL OF BONE AND MINERAL RESEARCH, 32, S133 - S133
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Structural Basis for Plexin Activation and Regulation
Journal article
Kong Y. et al, (2016), Neuron, 91, 548 - 560
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Next generation sequencing reveals a novel mutation in the XY-linker region of phospholipase C zeta (PLC zeta), resulting in truncated protein and oocyte activation deficiency
Conference paper
Amdani SN. et al, (2016), HUMAN REPRODUCTION, 31, 170 - 170
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Production of Heteromeric Transmembrane Receptors with Defined Subunit Stoichiometry
Journal article
Malinauskas T. and Furukawa H., (2016), Structure, 24, 1008 - 1009
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Production of Heteromeric Transmembrane Receptors with Defined Subunit Stoichiometry
Journal article
Malinauskas T. and Furukawa H., (2016), Structure, 24, 653 - 655
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Tailoring a Combination Preerythrocytic Malaria Vaccine
Journal article
Bauza K. et al, (2016), Infection and Immunity, 84, 622 - 634
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Genetic Control over mtDNA and Its Relationship to Major Depressive Disorder
Journal article
Cai N. et al, (2015), Current Biology, 25, 3170 - 3177
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Application of whole genome and RNA sequencing to investigate the genomic landscape of common variable immunodeficiency disorders
Journal article
van Schouwenburg PA. et al, (2015), Clinical Immunology, 160, 301 - 314
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Extracellular modulators of Wnt signalling
Journal article
Malinauskas T. and Jones EY., (2014), Current Opinion in Structural Biology, 29, 77 - 84
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Genomes and phenomes of a population of outbred rats and its progenitors
Journal article
Baud A. et al, (2014), Scientific Data, 1
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Structural insights into the inhibition of Wnt signaling by cancer antigen 5T4/Wnt-activated inhibitory factor 1
Journal article
Zhao Y. et al, (2014), Structure, 22, 612 - 620
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Structural Insights into the Inhibition of Wnt Signaling by Cancer Antigen 5T4/Wnt-Activated Inhibitory Factor 1
Journal article
Zhao Y. et al, (2014), Structure, 22, 612 - 620
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Efficacy of a Plasmodium vivax Malaria Vaccine Using ChAd63 and Modified Vaccinia Ankara Expressing Thrombospondin-Related Anonymous Protein as Assessed with Transgenic Plasmodium berghei Parasites
Journal article
Bauza K. et al, (2014), Infection and Immunity, 82, 1277 - 1286
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Abstract
Journal article
(2013)
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Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling
Journal article
Nachtergaele S. et al, (2013), eLife, 2
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Structural insights into proteoglycan-shaped Hedgehog signaling
Journal article
Whalen DM. et al, (2013), Proceedings of the National Academy of Sciences, 110, 16420 - 16425
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Combined sequence-based and genetic mapping analysis of complex traits in outbred rats
Journal article
(2013), Nature Genetics, 45, 767 - 775
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Neuropilins Lock Secreted Semaphorins onto Plexins in a Ternary Signalling Complex
Conference paper
Malinauskas T. et al, (2013), Biophysical Journal, 104, 613a - 613a
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Neuropilins lock secreted semaphorins onto plexins in a ternary signaling complex
Journal article
Janssen BJC. et al, (2012), Nature Structural & Molecular Biology, 19, 1293 - 1299
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Correction
Journal article
(2012), Biophysical Journal, 102, 1468 - 1468
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High-throughput Molecular Docking Now in Reach for a Wider Biochemical Community
Conference paper
Balan DM. et al, (2012), 2012 20th Euromicro International Conference on Parallel, Distributed and Network-based Processing
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Modular Mechanism of Wnt Signalling Inhibition by Wnt Inhibitory Factor 1
Journal article
Malinauskas T. et al, (2012), Biophysical Journal, 102, 518a - 518a
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Structural and Functional Studies of LRP6 Ectodomain Reveal a Platform for Wnt Signaling
Journal article
Chen S. et al, (2011), Developmental Cell, 21, 848 - 861
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Modular mechanism of Wnt signaling inhibition by Wnt inhibitory factor 1
Journal article
Malinauskas T. et al, (2011), Nature Structural & Molecular Biology, 18, 886 - 893
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Modular mechanism of Wnt signaling inhibition by Wnt inhibitory factor 1
Journal article
Malinauskas T. et al, (2011), Nature Structural and Molecular Biology, 18, 886 - 893
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Docking of Fatty Acids into the WIF Domain of the Human Wnt Inhibitory Factor-1
Journal article
Malinauskas T., (2008), Lipids, 43, 227 - 230
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Prussian blue-based amperometric sensor as a tool for the study of Pichia pastoris alcohol oxidase-catalyzed reactions
Journal article
Malinauskas T. and Malinauskas A., (2005), BULLETIN OF ELECTROCHEMISTRY, 21, 289 - 295