Seminar Dr Lori Passmore: "Mechanistic and structural insights into protein complexes that regulate DNA repair and mRNA stability"
Friday, 05 July 2019, 11am to 12pm
Venue: WCHG, Rooms A & B.
Dr Lori Passmore is from MRC Laboratory of Molecular Biology. More information about her can be found at this link
https://www2.mrc-lmb.cam.ac.uk/group-leaders/n-to-s/lori-passmore/
Abstract: Almost every eukaryotic mRNA has a poly(A) tail which is added by a 1 MDa multi-protein complex called Cleavage and Polyadenylation Factor (CPF/CPSF). There are four different enzymes in CPF (nuclease, polymerase and two protein phosphatases) but it is not understood how these are co-ordinated to define mRNA 3′-ends and regulate transcription. In addition, it is not clear what makes the poly(A) RNA sequence unique. We use a combination of structural biology (cryo-EM, X-ray crystallography, NMR) and biochemical reconstitution to address these questions and to gain mechanistic insight.