SINGLE PARTICLE ELECTRON MICROSCOPY OF THE MITOCHONDRIAL ATP SYNTHASE
BAKER LA., RUBINSTEIN JL.
Mitochondrial ATP synthase is a large, membrane-bound protein complex that plays a central role in cellular metabolism. Since the identification of this assembly in micrographs of mitochondrial membranes, electron microscopy has been crucial in elucidating the structure and mechanism of the enzyme. This review addresses the recent use of single particle electron microscopy for structure determination of ATP synthase, including subunit localization, the challenges posed by the protein, and areas in which further work is needed.