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In this chapter, we describe production and purification of the Zika virus NS1 glycoprotein in human embryonic kidney (HEK293T) cells at small, research laboratory scale. The expression of secreted NS1 (sNS1) and the C-terminal β-ladder domain in HEK293T cells were tested in a small-scale transfection before scaling up to a larger-scale transfection using roller bottles. Two different purification approaches have been applied to obtain purified NS1 (sNS1) and the C-terminal β-ladder domain ready for clinical applications.

Original publication




Journal article


Methods in molecular biology (Clifton, N.J.)

Publication Date





93 - 102


The Jenner Institute, The Henry Wellcome Building for Molecular Physiology, Nuffield Department of Medicine, University of Oxford, Oxford, UK.


Humans, Histidine, Oligopeptides, Recombinant Proteins, Viral Nonstructural Proteins, Serologic Tests, Cell Culture Techniques, Cloning, Molecular, Transfection, Proteomics, Secretory Pathway, HEK293 Cells, Zika Virus