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Foot-and-mouth disease vaccination using inactivated virus is suboptimal, as the icosahedral viral capsids often disassemble into antigenically distinct pentameric units during long-term storage, or exposure to elevated temperature or lowered pH, and thus raise a response that is no longer protective. Furthermore, as foot-and-mouth disease virus (FMDV)'s seven serotypes are antigenically diverse, cross-protection from a single serotype vaccine is limited, and most existing mouse and bovine antibodies and camelid single-domain heavy chain-only antibodies are serotype-specific. For quality control purposes, there is a real need for pan-serotype antibodies that clearly distinguish between pentamer (12S) and protective intact FMDV capsid. To date, few cross-serotype bovine-derived antibodies have been reported in the literature. We identify a bovine antibody with an ultralong CDR-H3, Ab117, whose structural analysis reveals that it binds to a deep, hydrophobic pocket on the interior surface of the capsid via the CDR-H3. Main-chain and hydrophobic interactions provide broad serotype specificity. ELISA analysis confirms that Ab117 is a novel pan-serotype and conformational epitope-specific 12S reagent, suitable for assessing capsid integrity.

Original publication

DOI

10.1099/jgv.0.002032

Type

Journal

The Journal of general virology

Publication Date

10/2024

Volume

105

Addresses

The Division of Structural Biology, Nuffield Department of Medicine, The Centre for Human Genetics, University of Oxford, Oxford, OX3 7BN, UK.

Keywords

Animals, Cattle, Foot-and-Mouth Disease Virus, Capsid, Foot-and-Mouth Disease, Capsid Proteins, Antibodies, Viral, Epitopes, Cross Reactions, Serogroup