African swine fever virus is a cytolytic virus that leads to the apoptosis of both cultured cells and primary macrophages. Cell culture supernatants of virus-infected cells are routinely used for virological and immunological studies, despite differences in the biological behavior between such preparations and highly purified virus. In addition, more recent data suggests that exosomes containing viral proteins may be secreted from infected cells. While African swine fever virus can be purified through a number of methods, in our hands Percoll provides the most robust method of separating virus from cellular contaminants.
Methods in molecular biology (Clifton, N.J.)
179 - 186
African Swine Fever Vaccinology Group, The Pirbright Institute, Pirbright, Woking, UK.
Cells, Cultured, Cell Line, Animals, Swine, DNA Viruses, African Swine Fever Virus, African Swine Fever, Viral Proteins