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The bacterial outer membrane proteins OmpA and OmpX were modified in such a manner that they yielded bulky crystals diffracting X-rays isotropically beyond 2 A resolution and permitting detailed structural analyses. The procedure involved semi-directed mutagenesis, mass production into inclusion bodies, and (re)naturation therefrom; it should be applicable for a broader range of membrane proteins.

Original publication

DOI

10.1002/(sici)1097-0134(19990201)34:2<167::aid-prot2>3.0.co;2-h

Type

Journal article

Journal

Proteins

Publication Date

02/1999

Volume

34

Pages

167 - 172

Addresses

Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität, Freiburg im Breisgau, Germany.

Keywords

Inclusion Bodies, Escherichia coli, Hydrolases, Bacterial Outer Membrane Proteins, Escherichia coli Proteins, Crystallization, Crystallography, X-Ray, Mutagenesis, Protein Denaturation