Strategy for membrane protein crystallization exemplified with OmpA and OmpX.
Pautsch A., Vogt J., Model K., Siebold C., Schulz GE.
The bacterial outer membrane proteins OmpA and OmpX were modified in such a manner that they yielded bulky crystals diffracting X-rays isotropically beyond 2 A resolution and permitting detailed structural analyses. The procedure involved semi-directed mutagenesis, mass production into inclusion bodies, and (re)naturation therefrom; it should be applicable for a broader range of membrane proteins.