Histamine Scavenging Attenuates Endotoxin‐Induced Acute Lung Injury
RYFFEL B., COUILLIN I., MAILLET I., SCHNYDER B., PAESEN GC., NUTTALL P., WESTON‐DAVIES W.
Histamine is an important mediator of early and late inflammatory responses. Here we asked whether scavenging of endogenous histamine by the arthropod‐derived histamine binding protein EV131 diminishes acute respiratory distress syndrome (ARDS) induced by inhaled endotoxin. We demonstrate that EV131 (360 μg given intranasally) reduced endotoxin‐induced bronchoconstriction and recruitment of neutrophils. Furthermore, EV131 administration diminished TNF‐α and protein leak in the bronchoalveolar lavage fluid. The data suggest that histamine attenuates endotoxin‐induced bronchoconstriction and neutrophil recruitment. Therefore, scavenging of histamine by EV131 may represent a novel therapeutic strategy in ARDS.