Strubi Profile

Joanne Nettleship

Post Doctoral Research Associate

Summary

I am a senior scientist working for the Oxford Protein Production Facility – UK, part of the Nuffield Department of Medicine, Oxford University. A chemist by training, I received my first degree from Oxford University followed by a PhD in Biological Chemistry from Bristol University. Over the last ten years, I have contributed towards the development of methodologies for the high-throughput production of proteins for crystallization studies. In particular, I have focussed on protein purification, characterization and crystallization using E. coli, insect and mammalian cells. I have developed the use of mammalian cell lines for production of recombinant glycoproteins and published many of these protocols. In addition, I have taught high-throughput laboratory techniques to visitors from both UK and European laboratories.

Responsibilities

I am part of a team involved in the development and running of a robust high-throughput protein production pipeline for structure determination by X-ray crystallography. I have responsibility for scale-up, protein purification and quality assurance of proteins produced using E. coli and eukaryotic cell lines. This includes management of internal projects alongside those from external clients, and development of new methodologies. I have responsibility for the line-management of postgraduate research assistants within the scale-up and purification team. I am particularly involved in development of technologies associated with production of proteins using mammalian and insect cells. I have implemented methodology using ESI-MS for the examination of selenomethionine incorporation in proteins, analysis of crystal-derived protein samples and analysis of glycosylation site occupancy. I am involved in construct design, ligation-independent cloning experiments and expression screening of recombinant proteins using both E. coli and eukaryotic cell lines. I have taken many projects through automated crystallization trials and with collaborators solved the X-ray crystal structures of these proteins.