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Graphene Oxide Grid Protocols

A couple of potentially helpful protocols.

Processing using Relion3.0/Motioncorr

Publications

Publication 1 is the one that we have tested the most, and works with the GP2 parameters described below. If you try it out, we would really appreciate your feedback!

 

1. https://www.biorxiv.org/content/10.1101/2021.03.08.434344v1

Efficient graphene oxide coating improves cryo-EM sample preparation and data collection from tilted grids

Avinash Patel, Daniel Toso, Audrey Litvak, Eva Nogales
2. DOI: 10.1038/ncomms11293 

Cryo-EM structure of lysenin pore elucidates
membrane insertion by an aerolysin family protein
Monika Bokori-Brown, Thomas G. Martin, Claire E. Naylor, Ajit K. Basak, Richard W. Titball, Christos G. Savva

3. doi:10.1016/j.jsb.2010.10.002 
Graphene: Substrate preparation and introduction
Radosav S. Pantelic a, Ji Won Suk b, Carl W. Magnuson b, Jannik C. Meyer c, Philipp Wachsmuth c, Ute Kaiser c,

Rodney S. Ruoff b, Henning Stahlberg

GP2 Settings

Thus far, Loic has found optimal results when plunging his grids using the leica plunger and has had consistent success with a range of samples now. His settings are saved on the Leica for all to use with 3 uL front, 1 uL back.