Graphene Oxide Grid Protocols
A couple of potentially helpful protocols.
Processing using Relion3.0/Motioncorr
Publication 1 is the one that we have tested the most, and works with the GP2 parameters described below. If you try it out, we would really appreciate your feedback!
Efficient graphene oxide coating improves cryo-EM sample preparation and data collection from tilted grids
Cryo-EM structure of lysenin pore elucidates
membrane insertion by an aerolysin family protein
Monika Bokori-Brown, Thomas G. Martin, Claire E. Naylor, Ajit K. Basak, Richard W. Titball, Christos G. Savva
Rodney S. Ruoff b, Henning Stahlberg
Thus far, Loic has found optimal results when plunging his grids using the leica plunger and has had consistent success with a range of samples now. His settings are saved on the Leica for all to use with 3 uL front, 1 uL back.